第59期：日糧添加三丁酸甘油酯對成年短尾母羊的瘤胃微生物蛋白質產量，發酵特性及...

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作者：Qing-Chang Ren1，Jing-Jing Xuan1，Li-Ke Wang1，Qiu-Wen Zhan1，Ding-Zhong Yin1，Zhong-Ze Hu1，Hong-Jian Yang2，Wei Zhang2，Lin-Shu Jiang3

1Anhui Science and Technology University,Fengyang, China

2State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University (CAU), Beijing,China

3Beijing Key Laboratory of Dairy CowNutrition, Beijing University of Agriculture,Beijing, China

來源：Anim Sci J. 2018;1–9.DOI: 10.1111/asj.13033

翻譯：腸動力研究院 梁琦

摘 要

本研究旨在通過體外和體內試驗來評估日糧添加三丁酸甘油酯（TB）對成年短尾母羊的瘤胃微生物蛋白質產量和發酵特性的影響。在體外試驗中，選取9只瘤胃插管的短尾母羊（12月齡，55±5kg），日糧以干物質（DM）為基礎，同時添加不同濃度的TB（0,2,4,6和8g / kg），二者混合48h，采食過后收集9只羊的瘤胃液用于分析TB對羊瘤胃養分降解率和發酵特性的影響。而在體內試驗中，45只成年母羊（12月齡，55±5kg）根據初始體重被隨機分配成5個試驗組，分飼DM基礎日糧+不同濃度的TB（0,2,4,6和8g / kg），每組9只，試驗為期18天。實驗結果顯示，體外試驗下，日糧添加TB顯著增強了成年母羊對DM（p=0.009），粗蛋白（p <0.001），中性洗滌纖維（p=0.007）和酸性洗滌纖維（p=0.010）的降解和利用，同時瘤胃的產甲烷能力相應增加（p <0.001）。而體內試驗則表明，日糧添加TB雖然顯著降低了成年母羊對DM的攝入量（p <0.001），但它顯著增加了瘤胃微生物蛋白質的生成（p <0.001）。總之，體外和體內試驗均表明日糧添加TB可使羊瘤胃內總揮發性脂肪酸（VFA）的濃度和纖維素酶（木聚糖酶，羧甲基纖維素酶，結晶纖維素酶）的活性得到相應的增加。研究結果表明，日糧添加TB可對羊瘤胃微生物蛋白質的產量和發酵特性產生積極的影響。

關鍵詞：成年母羊；甲烷；微生物粗蛋白；三丁酸甘油酯；揮發性脂肪酸

以下為相關圖表

表1：基礎日糧的組成及化學成分

表2：與干物質基礎日糧混合48h后，三丁酸甘油酯對體外發酵特性的影響

表3：體外試驗下，三丁酸甘油酯對干物質（DM），粗蛋白（CP），中性洗滌纖維（NDF）和酸性洗滌纖維（ADF）表觀降解率的影響

表4：體外試驗下，與干物質混合48小時后，三丁酸甘油酯對瘤胃產氣特性的影響

表5：體內和體外試驗下，三丁酸甘油酯對纖維素酶活性的影響

表6：三丁酸甘油酯對成年短尾母羊的日干物質攝入量和瘤胃內發酵特性的影響

表7：三丁酸甘油酯成年短尾母羊瘤胃內微生物繁殖的影響

結 論

雖然體外試驗未能減少產甲烷作用，但日糧添加三丁酸甘油酯（TB）對總VFA濃度，纖維素酶活性，發酵效率和微生物蛋白質合成具有有利影響，TB可能對成年母羊的生產性能產生積極的影響。

Abstract

Two trials were conducted to assess the effects of tributyrin (TB) supplementation on ruminal microbial protein yield and fermentation characteristics in adult sheep. In an in vitro trial, substrate was made to offer TB at 0, 2, 4, 6, and 8 g/kg on a dry matter (DM) basis and incubated for 48 hr. In an in vivo trial, 45 adult ewes were randomly assigned by initial body weight (55±5 kg) to five treatments of nine animals over an 18-day period. Total mixed ration was made to offer TB to ewes at 0, 2, 4, 6, and 8 g/kg on a DM basis. The in vitro trial showed that TB enhanced apparent degradation of DM (p = .009), crude protein (p < .001), neutral detergent fiber (p = .007) and acid detergent fiber (p = .010) and increased methanogenesis (p < .001), respectively. The in vivo trial showed that TB decreased DM intake (p < .001) and enhanced rumen microbial N synthesis (p < .001), respectively. Both in vitro and in vivo trials showed that TB increased total volatile fatty acid concentration and enhanced fibrolytic enzyme activity. The results indicated that TB might exert positive effects on microbial protein yield and fermentation in the rumen.

Conclusion

Although the in vitro trial failed to decrease methanogenesis, substrate and feed supplementation of TB showed advantageous effects on total VFA concentration, fibrolytic enzyme activity, fermentation efficiency and microbial protein synthesis, which might have positive effects on performance in adult ewes

原創： 梁琦      轉自：腸動力